Recipe

Buffer L2

Reagent Stock solution Quantity (for 50 mL) Final concentration
NaCl   5 m   2 mL 200 mm
EDTA (pH 8.0)  0.5 m  100 µL   1 mm
EGTA (pH 8.0)  0.5 m   50 µL  0.5 mm
Tris (pH 8.0)   1 m   500 µL  10 mm
dH2O 47.35 mL
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  1. doi:10.1101/pdb.rec083048 Cold Spring Harb Protoc 2018: pdb.rec083048- © 2018 Cold Spring Harbor Laboratory Press

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