Recipe

Oocyte Stripping Solution

Reagent Quantity Final concentration
N-methyl-d-glucamine 39.044 g 200 mm
Aspartate  26.62 g 200 mm
HEPES  4.766 g  20 mm
KCl 0.1491 g   2 mm
MgCl2·6H2O 0.2033 g   1 mm
EGTA  3.804 g  10 mm
H2O   to 1 L
Use 4 m NaOH to adjust the pH to 7.4. Store for 2 years at 4°C.
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  1. doi:10.1101/pdb.rec103788 Cold Spring Harb Protoc 2018: pdb.rec103788- © 2018 Cold Spring Harbor Laboratory Press
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