Pipette Solution for Patch Clamps
| Reagent | Quantity | Final concentration |
|---|---|---|
| KOH | 7.854 g | 140 mm |
| HEPES | 4.766 g | 20 mm |
| KCl | 0.1491 g | 2 mm |
| MgCl2·6H2O | 0.4066 g | 2 mm |
| Methanesulfonic acid | 13.314 g | 140 mm |
| H2O | to 1 L | |
| Use methanesulfonic acid to adjust pH to 7.1–7.2. Store no longer than 18 mo at room temperature (22°C–24°C). | ||










