Oocyte Stripping Solution
| Reagent | Quantity | Final concentration |
|---|---|---|
| N-methyl-d-glucamine | 39.044 g | 200 mm |
| Aspartate | 26.62 g | 200 mm |
| HEPES | 4.766 g | 20 mm |
| KCl | 0.1491 g | 2 mm |
| MgCl2·6H2O | 0.2033 g | 1 mm |
| EGTA | 3.804 g | 10 mm |
| H2O | to 1 L | |
| Use 4 m NaOH to adjust the pH to 7.4. Store for 2 years at 4°C. | ||










