Recipe

Pipette Solution for Patch Clamps

Reagent Quantity Final concentration
KOH  7.854 g 140 mm
HEPES  4.766 g  20 mm
KCl 0.1491 g   2 mm
MgCl2·6H2O 0.4066 g   2 mm
Methanesulfonic acid 13.314 g 140 mm
H2O   to 1 L
Use methanesulfonic acid to adjust pH to 7.1–7.2. Store no longer than 18 mo at room temperature (22°C–24°C).
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  1. doi:10.1101/pdb.rec103796 Cold Spring Harb Protoc 2018: pdb.rec103796- © 2018 Cold Spring Harbor Laboratory Press

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