The Basic Polymerase Chain Reaction (PCR)
Abstract
This protocol describes the reagents and procedures required to amplify a segment of double-stranded DNA in a chain reaction catalyzed by a thermostable DNA polymerase. It is the foundation for all subsequent variations of the polymerase chain reaction (PCR).
Footnotes
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From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.
