Recipe

Polyacrylamide Gel Solution (8% in 1×TBE)

Reagent Quantity (for 35 mL)
Acrylamide:bis-acrylamide (29:1) (40%)   7 mL
TBE buffer (10×)  3.5 mL
dH2O 23.51 mL
Ammonium persulfate (10%)   175 µL
TEMED   35 µL
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  1. doi:10.1101/pdb.rec083139 Cold Spring Harb Protoc 2018: pdb.rec083139- © 2018 Cold Spring Harbor Laboratory Press
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