Recipe

PBT for Xenopus Oocyte FISH

1× phosphate-buffered saline (PBS)

0.2 mg/mL bovine serum albumin (Sigma-Aldrich A2153)

0.1% (v/v) Triton X-100 (Fisher Scientific BP151)

Mix thoroughly. Sterilize through a 0.22-µm filter. Store at 4°C.

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  1. doi:10.1101/pdb.rec103630 Cold Spring Harb Protoc 2018: pdb.rec103630- © 2018 Cold Spring Harbor Laboratory Press
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