Protocol

In Vitro Fertilization in Mice

  1. Naomi Nakagata

Abstract

The success of in vitro fertilization is strongly influenced by genetic background, handling of sperm and oocytes, and culture conditions. This protocol promotes enhanced rates of fertilization by using preincubation medium for sperm containing methyl-β-cyclodextrin (MBCD) and fertilization medium with high calcium and reduced glutathione (GSH).

Footnotes

  • From the Manipulating the Mouse Embryo collection, edited by Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, and Andras Nagy.

Articles citing this article

| Table of Contents

This Article

  1. Published in Advance April 18, 2018, doi: 10.1101/pdb.prot094524 Cold Spring Harb Protoc 2018: pdb.prot094524- © 2018 Cold Spring Harbor Laboratory Press
  1. » Abstract
  2. Full Text
  3. Full Text (PDF)
  4. All Versions of this Article:
    1. pdb.prot094524v1
    2. 2018/6/pdb.prot094524 most recent

Article Category

  1. Protocol

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Subject Categories

  1. Mouse
  2. Developmental Biology

Share

Current Issue

  1. January 2026, 2026 (1)
  1. Current Issue