Recipe

Dejelly Solution #2 for X. tropicalis Eggs and X. laevis Embryos

Combine 15 g of L-cysteine (e.g., Sigma-Aldrich C-7352) with 450-mL MilliQ H2O. Adjust the pH to 7.8 with NaOH, and bring to 0.5 L (to make a 3% l-cysteine solution). Make fresh on day of prep and store at room temperature until use.

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  1. doi:10.1101/pdb.rec103515 Cold Spring Harb Protoc 2018: pdb.rec103515- © 2018 Cold Spring Harbor Laboratory Press

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