Dejelly Solution #2 for X. tropicalis Eggs and X. laevis Embryos
Combine 15 g of L-cysteine (e.g., Sigma-Aldrich C-7352) with 450-mL MilliQ H2O. Adjust the pH to 7.8 with NaOH, and bring to 0.5 L (to make a 3% l-cysteine solution). Make fresh on day of prep and store at room temperature until use.










