Energy Mix (50×)
| Reagent | Quantity |
|---|---|
| Creatine phosphate disodium salt tetrahydrate (MW 327.14) | 0.622 g |
| Adenosine 5′-triphosphate (ATP) disodium salt hydrate (MW 511.14) | 0.138 g |
| EGTA stock (K salt; 0.5 m; pH 7.7; filter-sterilized and stored in aliquots at –20°C) | 0.05 mL |
| MgCl2 stock (2 m; e.g., Sigma-Aldrich 68475) | 0.125 mL |
| Combine with MilliQ H2O to a volume of 10 mL. Make 0.1-mL aliquots, and store at −20°C. | |
