Marc's Modified Ringer's (MMR; 20×)
| Reagent | Quantity | Final concentration |
|---|---|---|
| HEPES, free acid | 95.32 g | 100 mm |
| EDTA | 2.98 g | 2 mm |
| NaCl | 467.52 g | 2 m |
| KCl | 11.93 g | 40 mm |
| CaCl2 | 23.52 g | 40 mm |
| MgCl2 (e.g., Sigma-Aldrich 68475) | 40 mL of 2 m stock | 20 mm |
| Combine with MilliQ H2O, adjust pH to 7.8–7.9 using ∼8.5 g of NaOH pellets, and bring to 4 L. Autoclave and store at room temperature. | ||
