Recipe

Sperm Dilution Buffer (SDB)

10 mm HEPES-KOH pH 7.8

1 mm MgCl2

100 mm KCl

150 mm sucrose

Store at −20°C in 1-mL aliquots.

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  1. doi:10.1101/pdb.rec103622 Cold Spring Harb Protoc 2018: pdb.rec103622- © 2018 Cold Spring Harbor Laboratory Press
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