Recipe

Nuclear Preparation Buffer (NPB) (1×)

Sucrose (2 m)  25 mL
HEPES (1 m, pH 7.4)  3 mL
EDTA (0.5 m, pH 8.0) 400 µL
S/S Buffer (20×)  10 mL
Add ddH2O to 200 mL. Make fresh, and add DTT to 1 mm and LPC protease inhibitor cocktail (10 mg/mL leupeptin, pepstatin, and chymostatin stock in DMSO; EMD Millipore) at 1/1000 before use.
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  1. doi:10.1101/pdb.rec103887 Cold Spring Harb Protoc 2018: pdb.rec103887- © 2018 Cold Spring Harbor Laboratory Press
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