Topic Introduction

Gateway-Compatible Yeast One-Hybrid and Two-Hybrid Assays

  1. Albertha J.M. Walhout

Abstract

In the first section of this introduction, we provide background information for yeast two-hybrid (Y2H) assays that provide a genetic method for the identification and analysis of binary protein–protein interactions and that are complementary to biochemical methods such as immunoprecipitation. In the second section, we discuss yeast one-hybrid (Y1H) assays that provide a “gene-centered” (DNA-to-protein) genetic method to identify and study protein–DNA interactions between cis-regulatory elements and transcription factors (TFs). This method is complementary to “TF-centered” (protein-to-DNA) biochemical methods such as chromatin immunoprecipitation.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

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This Article

  1. doi:10.1101/pdb.top094953 Cold Spring Harb Protoc 2018: pdb.top094953- © 2018 Cold Spring Harbor Laboratory Press
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