Table 1.

Y2H DB-bait selection

Y2H bait	Example	Advantages	Disadvantages
Full-length protein	Any of the proteins encoded by a genome	Interaction can likely function in relevant biological context	Higher false-negative rate; full-length protein may be autoactivator
Protein domain(s)	Either consecutive protein fragments or specific domains can be selected	Lower false-negative rate	Interactions may not occur with full-length protein More screens are required per protein