Table 2.

Y2H AD-prey selection

Y2H prey Requirements Advantages Disadvantages Cost Throughput
cDNA library High complexity; relevant to tissue of interest Prey of choice when ORF collections are not available Difficult to detect low-abundant proteins High Low
ORFeome library Enough ORFs, cloned into the AD-prey vector Can detect low-abundant proteins Cannot find uncloned proteins Medium Medium
ORF smart pools Enough ORFs, cloned into the AD-prey vector Can detect low-abundant proteins Cannot find uncloned proteins Medium Medium/high
ORF arrays Enough ORFs, cloned into the AD-prey vector Can detect low-abundant proteins Cannot find uncloned proteins Low High

  • Y2H, yeast two-hybrid; AD, activation domain; ORF, open reading frame.

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