Table 3.
Y1H DNA-bait selection
| Y1H bait | Example | Length | Cloning | Advantages | Disadvantages |
|---|---|---|---|---|---|
| Small cis-regulatory element | Predicted TF binding site | 5–12 bp, one to five copies in tandem | Annealing of ds oligonucleotides | Low background | Need prior information |
| Large, composite cis-regulatory fragment | Promoter/enhancer | ∼50–2000 bp | PCR from genomic DNA | Unbiased | Can have higher background (∼10%–20% of fragments) |
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Y1H, yeast one-hybrid; TF, transcription factor; ds, double-stranded; PCR, polymerase chain reaction.
