Table 4.
Y1H AD-prey selection
| Y1H prey | Requirements | Advantages | Disadvantages | Cost | Throughput |
|---|---|---|---|---|---|
| cDNA library | High complexity; relevant to tissue of interest | Can find uncloned or unpredicted TFs | Difficult to detect TFs that are expressed at low levels, in only a few cells, or restricted (developmental) periods | High | Low |
| TF minilibrary | Enough TF-encoding ORFs, cloned into the AD-prey vector | Can detect low-abundance TFs | Cannot find uncloned TFs | Medium | Medium |
| TF smart pools | Enough TF-encoding ORFs, cloned into the AD-prey vector | Can detect low-abundance TFs | Cannot find uncloned TFs | Medium | Medium/high |
| TF array | Enough TF-encoding ORFs, cloned into the AD-prey vector | Can detect low-abundance TFs | Cannot find uncloned TFs | Low | High |
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Y1H, yeast one-hybrid; AD, activation domain; TF, transcription factor; ORF, open reading frame.
