Formamide Loading Buffer for RNA Gels
| Deionized formamide | 80% (w/v) |
| EDTA (pH 8.0) | 10 mm |
| Xylene cyanol FF | 1 mg/mL |
| Bromophenol blue | 1 mg/mL |
| Purchase a distilled deionized preparation of formamide and store in small aliquots under nitrogen at −20°C.Alternatively, deionize reagent-grade formamide by stirring on a magnetic stirrer with Dowex XG8 mixed bed resin for 1 h and filtering it twice through Whatman No. 1 paper. Store deionized formamide in small aliquots under nitrogen at −70°C. | |
