Recipe

3C Lysis Buffer

Reagent Stock solution Quantity (for 100 mL) Final concentration
Tris-HCl (pH 8.0)  1 m    1 mL   10 mm
NaCl  1 m    1 mL   10 mm
H2O  97.8 mL
Igepal CA-630 100%  200 µL 0.2% (v/v)
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  1. doi:10.1101/pdb.rec099077 Cold Spring Harb Protoc 2018: pdb.rec099077- © 2018 Cold Spring Harbor Laboratory Press
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