Recipe

Calcium Ionophore Solution

Add 5 μL of calcium ionophore A23187 (Sigma-Aldrich C7522; 10 mg/mL stock in DMSO; stored at 4°C protected from light) to 100 mL of 0.2× Marc's modified Ringer's (MMR; from 20× stock). Prepare fresh and use immediately.

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  1. doi:10.1101/pdb.rec103937 Cold Spring Harb Protoc 2018: pdb.rec103937- © 2018 Cold Spring Harbor Laboratory Press
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