Egg Laying Buffer (20×)
| Reagent | Concentration |
|---|---|
| NaCl | 2 m |
| KCl | 40 mm |
| MgSO4 | 20 mm |
| CaCl2 | 50 mm |
| HEPES (potassium salt; 1 m; pH 7.7 with KOH; filter-sterilized and stored at −20°C) | 10 mm |
| EDTA | 2 mm |
| Adjust pH of 20× buffer to 7.4 with 10 m NaOH. Store at room temperature, and dilute to 1× with frog tank water before use. | |
