Energy Reaction Mix (ER Mix)
| Reagent | Amount to add | Final concentration (20×) |
|---|---|---|
| Adenosine triphosphate (Sigma-Aldrich A2383) | 10.1 mg | 20 mm |
| MgCl2 (Fisher BP214) | 1.7 mg | 20 mm |
| Creatine phosphate (Sigma-Aldrich 2380) | 31.7 mg | 150 mm |
| Creatine phosphokinase (Sigma-Aldrich C3755) | 600 μg | 600 μg/mL |
| Dissolve in 1 mL of deionized water. Mix by vortexing. Store 50-μL aliquots frozen at −20°C for several months; for longer-term storage, maintain samples at −80°C. | ||
