| NaCl (Sigma-Aldrich, S9888; CAS No. 7647-14-5; purity 99.0%) |
0.625 g |
| NaHCO3 (Sigma-Aldrich, S8875; 144-55-8; purity 99.0%)
|
0.096 g |
| MgSO4 (Sigma-Aldrich, M7506; CAS No. 7487-88-9; purity 99.0%)
|
0.075 g |
| CaSO4 • 2 H2O (Sigma-Aldrich, C3771; CAS No. 10101-41-4; purity 99.0%)
|
0.06 g |
| KCl (Sigma-Aldrich, P3911; CAS No. 7447-40-7; purity 99.0%) |
0.03 g |
| CaCl2 • 2 H2O (Sigma-Aldrich, C5080; CAS No. 10035-04-8; purity 99.0%)
|
0.02 g |
| Dissolve in 1 L of deionized water. Store at 4°C. The final product is a reconstituted water medium (Dawson and Bantle 1987).
|
