Starvation Medium for [32P]Orthophosphate Labeling
| Reagent | Volume per 100 mL of solution (v/v) | Final concentration |
|---|---|---|
| Phosphate-free DMEM (1×) | 86.5–96.5 mL (depending on serum concentration) | 1× |
| Dialyzed calf serum | 0–10 mL | 0%–10% as required |
| l-Glutamine (100×, 200 mm) | 1 mL | 2 mm |
| HEPES-KOH (pH 7.4, 1 m) | 2.5 mL | 25 mm |
| Prepare fresh under sterile conditions. Warm to 37°C. | ||
