Chasing Medium for [35S]Methionine Labeling
| Reagent | Volume per 100 mL of solution | Final concentration |
|---|---|---|
| DMEM (1×) | 88 mL | 1× |
| l-Glutamine (100×, 200 mm) | 1 mL | 2 mm |
| l-Methionine (100×, 200 mm) | 1 mL | 2 mm |
| Calf serum | 10 mL | 10% |
| HEPES-KOH (pH 7.4, 1 m) | 2.5 mL | 25 mm |
| Additional methionine supplementation will assist in chasing any [35S]methionine remaining in the cells. Warm to 37°C. | ||
