Recipe

Ligase Reaction Buffer (10×)

Reagent Final concentration
Tris-HCl (pH 7.8)  500 mm
MgCl2  100 mm
Dithiothreitol  100 mm
ATP   10 mm
Bovine serum albumin 250 µg/mL
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  1. doi:10.1101/pdb.rec098830 Cold Spring Harb Protoc 2019: pdb.rec098830- © 2019 Cold Spring Harbor Laboratory Press
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