Recipe

SapI Reaction Buffer (10×)

Reagent Final concentration
Potassium acetate 500 mm
Tris-acetate 200 mm
Magnesium acetate 100 mm
Dithiothreitol  10 mm
Adjust pH to 7.9.
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  1. doi:10.1101/pdb.rec098848 Cold Spring Harb Protoc 2019: pdb.rec098848- © 2019 Cold Spring Harbor Laboratory Press
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