PCR Tissue Homogenization Buffer (10×)
| Reagent | Final concentration |
|---|---|
| KCl (1 m) | 500 mm |
| Tris-HCl | 100 mm |
| Gelatin (Sigma-Aldrich G2500) | 0.1 mg/mL |
| Nonidet P-40 (Sigma-Aldrich N6507) | 0.45% (v/v) |
| Tween 20 (Sigma-Aldrich P1379) | 0.45% (v/v) |
| Proteinase K | 500 µg/mL |
| Add the Proteinase K just before use (20 mg/mL stock). Adjust the pH to 8.3 before addition of gelatin and detergents; this buffer can be stored at room temperature in small aliquots. | |
