Recipe

GC-Rich Amplification Buffer (10×)

Reagent Quantity (for 10 mL) Final concentration
Ammonium sulfate (1 m) 1.66 mL   166 mm
MgCl2 (1 m)  300 µL   30 mm
Tris-HCl (1 m, pH 8.5)  6.6 mL   0.66 m
Tween 20   10 µL 0.1% (v/v)
Store at 4°C.
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  1. doi:10.1101/pdb.rec096685 Cold Spring Harb Protoc 2019: pdb.rec096685- © 2019 Cold Spring Harbor Laboratory Press
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