Additive Solution (5×)
| Reagent | Quantity (for 10 mL) | Final concentration |
|---|---|---|
| Betaine (5 m) | 5.4 mL | 2.7 m |
| Dithiothreitol (1 m) | 67 µL | 6.7 mm |
| Dimethyl sulfoxide | 670 µL | 6.7% (v/v) |
| Bovine serum albumin (2 mg/mL) | 275 µL | 55 µg/mL |
| Store at 4°C for no longer than a week. | ||
