Chromosome Dilution Buffer
| Reagent | Amount to add | Final concentration (1×) |
|---|---|---|
| K-HEPES (1 m, pH 7.6) | 1 mL | 10 mm |
| KCl (2 m) | 10 mL | 200 mm |
| MgCl2 (1 m) | 0.1 mL | 0.5 mm |
| K-EGTA (0.5 m) | 0.2 mL | 0.5 mm |
| Sucrose | 8.57 g | 250 mm |
| Mix and dissolve the reagents listed above in H2O to a final volume of 100 mL. Store in 10-mL aliquots at −20°C. | ||
