Recipe

Energy Mix (EM) (35×)

Reagent Amount to add Final concentration (35×)
ATP (200 mm)  10 µL   ∼67 mm
Creatine phosphate (phosphocreatine) (1 m)  20 µL  ∼667 mm
Creatine phosphokinase (5 mg/mL) 0.8 µL 0.13 mg/mL
Prepare fresh 35× EM by mixing the stock solutions as indicated. Keep on ice. Discard unused portion at the end of the experiment.
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  1. doi:10.1101/pdb.rec104257 Cold Spring Harb Protoc 2019: pdb.rec104257- © 2019 Cold Spring Harbor Laboratory Press
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