Dithiothreitol Solution (DTT, 1 m)
Dissolve 3.09 g of dithiothreitol in 20 mL of 0.01 m sodium acetate (pH 5.2) and sterilize by filtration. Dispense into 1-mL aliquots and store at −20°C. Under these conditions, dithiothreitol is stable to oxidation by air.
