Ficoll–Orange G Loading Buffer (6×)
| Reagent | Quantity (for 100 mL) | Final concentration (6×) |
|---|---|---|
| Ficoll-400 | 15 g | 15% (w/v) |
| Orange G | 250 mg | 0.25% (w/v) |
| H2O | to 100 mL | |
| Store in 1-mL aliquots at −20°C. | ||
| Reagent | Quantity (for 100 mL) | Final concentration (6×) |
|---|---|---|
| Ficoll-400 | 15 g | 15% (w/v) |
| Orange G | 250 mg | 0.25% (w/v) |
| H2O | to 100 mL | |
| Store in 1-mL aliquots at −20°C. | ||