T7 Transcription Buffer for DNA Template Preparation (10×)
| Reagent | Quantity (for 100 mL) | Final concentration (10×) |
|---|---|---|
| Tris-HCl (1 m, pH 7.9) | 40 mL | 400 mm |
| Spermidine (1 m) | 2.5 mL | 25 mm |
| MgCl2 (1 m) | 26 mL | 260 mm |
| Triton X-100 | 1 mL | 0.1% (w/v) |
| H2O | to 100 mL | |
| Store in 1-mL aliquots at −20°C. | ||
