Hoechst Stock Solution
| Reagent | Quantity (∼400 µL) | Final concentration |
|---|---|---|
| Sucrose (1 m) | 80 µL | 200 mm |
| HEPES-KOH (1 m, pH 7.6) | 4 µL | 10 mm |
| Formaldehyde (37%) | 80 µL | 7.4% |
| Hoechst (1.0 mg/mL) | 3.2 µL | 8 µg/mL |
| H2O | 232 µL | |
| Dilute 10 mg/mL stock of Hoechst in water prior to use above. Store final solution at room temperature protected from light. | ||
