Buffer X (10×)
| Reagent | Quantity (20 mL) | Final concentration |
|---|---|---|
| HEPES-KOH (1 m, pH 7.4–7.6) | 2 mL | 100 mm |
| KCl (1 m) | 1.6 mL | 80 mm |
| NaCl (5 m) | 0.6 mL | 150 mm |
| MgCl2 (1 m) | 1 mL | 50 mm |
| EDTA (0.5 m) | 0.4 mL | 10 mm |
| dH2O | 14.4 mL | |
| Make fresh on the day of extract preparation. | ||
