Marc's Modified Ringer's (MMR) for Xenopus (20×)
| Reagent | Quantity (1 L) | Final concentration |
|---|---|---|
| NaCl | 116.8 g | 2 m |
| KCl | 3 g | 40 mm |
| MgSO4·7H2O | 2.4 g | 10 mm |
| CaCl2·2H2O | 7.34 g | 50 mm |
| EDTA | 0.58 g | 2 mm |
| HEPES | 23.8 g | 100 mm |
| Add water to 1 L. Adjust pH of solution to 7.8 with KOH. Filter-sterilize, and store at 4°C. | ||
