Protocol

RNAi in Mammalian Cells by siRNA Duplex Transfection

  1. Phillip D. Zamore

Abstract

Long dsRNA cannot be used in most cultured mammalian cells because it triggers the interferon response, causing widespread changes in gene expression and apoptosis. This protocol describes a method for delivering into mammalian cells siRNA duplexes that are too short to elicit the sequence-nonspecific responses associated with long dsRNA.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

| Table of Contents

This Article

  1. doi:10.1101/pdb.prot097451 Cold Spring Harb Protoc 2019: pdb.prot097451- © 2019 Cold Spring Harbor Laboratory Press
  1. » Abstract
  2. Full Text
  3. Full Text (PDF)

Article Category

  1. Protocol

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Share

Current Issue

  1. April 2026, 2026 (4)
  1. Current Issue