Xenopus Embryo Lysis Buffer

50 mm Tris-HCl (pH 7.5)

100 mm NaCl

10 mm MgCl₂

1 mm dithiothreitol

5% glycerol

1% Nonidet P-40

complete, Mini Protease Inhibitor (1 tablet/10 mL; Roche 4693124001)

PhosStop Phosphatase Inhibitor (1 tablet/10 mL; Roche PHOSS-RO)

Prepare fresh. Keep on ice until use.

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