DNase I Stock Solution (10 U/µL)
| Reagent | Final concentration |
|---|---|
| Tris-HCl (1 m, pH 7.6) | 20 mm |
| NaCl (5 m) | 50 mm |
| MgCl2 (1 m) | 2 mm |
| CaCl2 (1 m) | 2 mm |
| Pefabloc SC PLUS (Roche 11873601001) (20 mg/mL) | 0.1 mg/mL |
| Dithiothreitol (1 m) | 1 mm |
| Glycerol (100%) | 50% |
| Combine the reagents listed above to prepare storage buffer. On ice, solubilize an entire bottle of deoxyribonuclease I (DNase I [Sigma-Aldrich D4527; 10,000 U]) with 1 mL of ice-cold storage buffer. Prepare 50- to 100-µL aliquots and store at −20°C. | |
