| Tris-HCl (1 m, pH 8.0)
|
10 mm |
| Sucrose (1 m)
|
0.3 m |
| MgCl2 (1 m)
|
5 mm |
| KCl (1 m)
|
25 mm |
| NaF (500 mm)
|
10 mm |
| β-glycerophosphate (1 m)
|
5 mm |
| Sodium pyrophosphate (100 mm)
|
5 mm |
| Spermine (500 mm)
|
0.5 mm |
| Spermidine (500 mm)
|
0.5 mm |
| Pefabloc SC PLUS (Roche 11873601001) (20 mg/mL) |
0.1 mg/mL |
| Dithiothreitol (1 m)
|
2 mm |
| Protease inhibitor tablet (cOmplete, Mini, EDTA-free) (Roche 04693159001) |
1 tablet for 10 mL |
| Iodixanol (60%) (OptiPrep Density Gradient Medium; Sigma-Aldrich D1556) |
20%; 25%; 30% |
| Prepare fresh and keep at 4°C. |
