Recipe

Stop Buffer for DNase I

Reagent Final concentration
Tris-HCl (1 m, pH 8.0)  50 mm
NaCl (5 m) 100 mm
SDS (20%)  0.10%
EDTA (0.5 m [pH 8.0]) 100 mm
Spermine (500 mm)  1 mm
Spermidine (500 mm)  0.3 mm
Prepare fresh and equilibrate to 37°C prior to use.
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  1. doi:10.1101/pdb.rec105908 Cold Spring Harb Protoc 2019: pdb.rec105908- © 2019 Cold Spring Harbor Laboratory Press
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