Recipe

Marc's Modified Ringer's (MMR) (10×, pH 7.8)

Reagent Concentration (10×)
NaCl   1 m
KCl 20 mm
CaCl2 20 mm
MgSO4 10 mm
HEPES 50 mm
EDTA  1 mm
Adjust pH to 7.8 and sterilize by autoclaving. Use the solution at 0.05×. Store for up to 1 yr at room temperature.
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  1. doi:10.1101/pdb.rec106278 Cold Spring Harb Protoc 2019: pdb.rec106278- © 2019 Cold Spring Harbor Laboratory Press

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