Lysis Buffer for Xenopus Hi-C
| Reagent | Amount to add (for 5 mL) | Final concentration |
|---|---|---|
| Tris-HCl (1 m, pH 7.5) | 250 µL | 50 mm |
| NaCl (5 m) | 10 µL | 10 mm |
| EDTA (0.5 m, pH 8.0) | 10 µL | 1 mm |
| SDS (10%) | 280 µL | 0.56% |
| ddH2O (sterile) | 4.4 mL | |
| Store for up to 1 yr at room temperature. Add 50 µL of 100× protease inhibitor cocktail (Sigma-Aldrich P8340) immediately before use. | ||
