Routes of Antigen Injection in Mice and Rats

Abstract

The route of injection used to induce an antigenic response in small rodents is based on a number of practical considerations, such as the volume to be delivered, the buffers and other components co-injected with the immunogen, and the rate at which one desires the immunogen to be released into the lymphatics or circulation. For mice, large volumes are only practical with intraperitoneal injections. If adjuvants or particulate matter are included in the injection, the immunogen should not be delivered intravenously.