Recipe

Annealing Buffer II (10×)

Reagent Final concentration
Tris-HCl (pH 7.4) 200 mm
MgCl2  20 mm
NaCl 500 mm
Autoclave and store at −20°C.
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  1. doi:10.1101/pdb.rec098855 Cold Spring Harb Protoc 2019: pdb.rec098855- © 2019 Cold Spring Harbor Laboratory Press
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